Cell Signaling Technology

Product Pathways - Vesicle Trafficking

Rabex-5 (D21F12) Rabbit mAb #7622

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Endogenous 60-80 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Rabex-5 (D21F12) Rabbit mAb recognizes endogenous levels of total Rabex-5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rabex-5 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Rabex-5 siRNA I (+), using Rabex-5 (D21F12) Rabbit mAb #7622 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Rabex-5 (D21F12) Rabbit mAb confirms silencing of Rabex-5 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Rabex-5 (D21F12) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a mouse Rabex-5 (variant 2) cDNA construct (+), using Rabex-5 (D21F12) Rabbit mAb.


Background

Rabex-5, also called RabGEF1 and RAP1, was identified as a guanine nucleotide exchange factor (GEF) for Rab5, a member of the Ras superfamily of small Rab GTPases (1). Rabex-5 generates the GTP-bound active form of Rab5 and forms a tight association with its effector protein Rabaptin-5 (2). This complex localizes to endosomal membranes where it functions as a key regulator of vesicular trafficking during early endocytosis (3,4). Rabex-5 is also monoubiquitinated and has ubiquitin ligase activity that regulates its recruitment to early endosomes (5,6). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis (5). Through its control of endosomal trafficking and endocytosis, Rabex-5 has been shown to negatively regulate NGF-mediated neurite outgrowth (7) as well as FcεRI-dependent mast cell activation (8).

  1. Horiuchi, H. et al. (1997) Cell 90, 1149-59.
  2. Lippé, R. et al. (2001) Mol Biol Cell 12, 2219-28.
  3. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
  4. van der Bliek, A.M. (2005) Nat Cell Biol 7, 548-50.
  5. Mattera, R. et al. (2006) J Biol Chem 281, 6874-83.
  6. Mattera, R. and Bonifacino, J.S. (2008) EMBO J 27, 2484-94.
  7. Liu, J. et al. (2007) Mol Biol Cell 18, 1375-84.
  8. Tam, S.Y. et al. (2004) Nat Immunol 5, 844-52.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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