Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Eg5 Antibody #7625

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 120 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Eg5 Antibody recognizes endogenous levels of total Eg5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Eg5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Eg5 Antibody.

Background

Eg5 (also called kinesin-like protein 11 or Kif11) belongs to the kinesin-like family of motor proteins important in chromosome positioning, centrosome separation, and mitotic spindle formation. Phosphorylation of Eg5 by mitotic kinases regulates its activity by modulating its association with microtubules (1,2). Because anti-mitotic chemotherapeutic drugs, such as taxanes, target microtubules and have pleiotropic and sometimes toxic effects, drugs that target microtubule-associated proteins such as Eg5 are currently in development (3-5).

  1. Blangy, A. et al. (1995) Cell 83, 1159-69.
  2. Rapley, J. et al. (2008) J Cell Sci 121, 3912-21.
  3. Sarli, V. and Giannis, A. (2006) ChemMedChem 1, 293-8.
  4. Cox, C.D. et al. (2008) J Med Chem 51, 4239-52.
  5. Nakai, R. et al. (2009) Cancer Res 69, 3901-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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