Product Pathways - MAPK Signaling
MAPKAPK-5 Kinase #7659
Cell Signaling Technology offers a full line of protein kinases, substrates, antibody detection reagents and HTScan® kits. Browse our "Reagents for High-Throughput Screening" product listing or contact us at drugdiscovery@cellsignal.com.
Description
Purified recombinant full length human MAPKAPK-5 kinase, supplied as a GST fusion protein.
Source / Purification
This GST-kinase fusion protein was produced using a baculovirus expression system with a construct expressing the kinase with an amino terminal GST tag. The protein was purified by one-step affinity chromatography using glutathione-agarose.
Quality Control
The purified kinase fusion protein was quality controlled for purity using SDS-PAGE followed by Coomassie or silver stain and Western blot. The specific activity of the kinase was determined using a radiometric assay.
Background
MAPKAPK5 belongs to the mitogen-activated protein kinase (MAPK) activated protein kinases (MK) subfamily that includes MAPKAPK2/MK2 and MK3/3pK. The MK subfamily is part of a family of protein kinase subfamilies downstream of MAPK pathways and includes ribosomal S6 kinase (RSKs), mitogen and stress activated kinases (MSKs) and MAPK-interacting kinases (MNKs). All MKs are activated by MAPK pathways and mediate important processes such as gene expression, cell-cycle progression and have been implicated in inflammation and cancer (1,2). MAPKAPK5 shows binding to and activation by the p38 kinase and extracellular-regulated kinases (ERK) (3,4). Recently, MAPKAPK5 was shown to be activated by ERK3 and act as a chaperone to ERK3 (5,6). While overexpressed MAPKAPK5 shares similar substrates with MAPKAPK2, such as HSP27 and glycogen synthase, recent work with MAPKAPK5 knock-out mice indicate distinct substrates and functional properties (7).
- Roux, P.P. and Blenis, J. (2004) Microbio.l Mol. Biol. Rev. 68, 320-344.
- Gaestel, M. (2006) Nat. Rev. Mol. Cell Biol. 7, 120-130.
- New, L. et al. (1998) EMBO J. 17, 3372-3384.
- Ni, H. et al. (1998) Biochem. Biophys. Res. Commun. 243, 492-496.
- Schumacher, S. et al. (2004) EMBO J. 23, 4770-4779.
- Seternes, O.M. et al. (2004) EMBO J. 23, 4780-4791.
- Shi, Y. et al. (2003) Mol. Cell Biol. 23, 7732-7741.
Application References
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Companion Products
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
