Product Pathways - Ca / cAMP / Lipid Signaling
S100P Antibody #7677
|7677S||100 µl (10 western blots)||---||In Stock||---|
|7677||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
S100P Antibody recognizes endogenous levels of total S100P protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp50 of human S100P protein. Antibodies are purified by protein A and peptide affinity chromatography.
Despite their relatively small size (8-12 kDa) and uncomplicated architecture, S100 proteins regulate a variety of cellular processes such as cell growth and motility, cell cycle progression, transcription, and differentiation. To date, 25 members have been identified, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z, making it the largest group in the EF-hand, calcium-binding protein family. Interestingly, 14 S100 genes are clustered on human chromosome 1q21, a region of genomic instability. Research studies have demonstrated that significant correlation exists between aberrant S100 protein expression and cancer progression. S100 proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development (1-4).
Each S100 monomer bears two EF-hand motifs and can bind up to two molecules of calcium (or other divalent cation in some instances). Structural evidence shows that S100 proteins form antiparallel homo- or heterodimers that coordinate binding partner proximity in a calcium-dependent (and sometimes calcium-independent) manner. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellular compartments, and display unique protein binding partners, which suggests that each plays a specific role in various signaling pathways. In addition to an intracellular role, some S100 proteins have been shown to act as receptors for extracellular ligands or are secreted and exhibit cytokine-like activities (1-4).
S100P, a member of the S100 family, is a 95 amino acid protein. Its expression is increased in a number of tumors including pancreas, lung, breast, and ovary carcinomas (5) in response to transcriptional activation (6). Abnormally high levels of S100P are thought to contribute to tumor development and metastatic properties (7).
- Heizmann, C.W. et al. (2002) Front Biosci 7, d1356-68.
- Donato, R. (2003) Microsc Res Tech 60, 540-51.
- Marenholz, I. et al. (2004) Biochem Biophys Res Commun 322, 1111-22.
- Santamaria-Kisiel, L. et al. (2006) Biochem J 396, 201-14.
- Jiang, H. et al. (2012) J Cancer Res Clin Oncol 138, 1-9.
- Gibadulinova, A. et al. (2008) Oncol Rep 20, 391-6.
- Arumugam, T. and Logsdon, C.D. (2011) Amino Acids 41, 893-9.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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