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Prestained Protein Marker, Broad Range (Premixed Format) #7720
Description
Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands between 6 and 175 kDa when electrophoresed (1). The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins. The apparent molecular weight of the prestained proteins are given in the table below.
Western Blotting
10?20% SDS-PAGE
Directions for Use
(a) Transfer the desired amount of the Prestained Protein Marker to a separate tube. For visualizing during electrophoresis: use 15 µl for mini-gels and 30 µl for full length gels.
(b) Heat the Marker to 95-100°C for 3-5 minutes.
(c) After a quick microcentrifuge spin, load directly onto a gel. To ensure uniform mobility, load an equal volume of 1X reducing SDS Loading Buffer into any unused wells. Prestained Protein Marker, Broad Range (Premixed Format) is stable for a minimum of 6 months at -20°C.
Prestained Protein Marker, Broad Range
| Protein | Source | Apparent MW (kDa) |
|---|---|---|
| MBP-β-galactosidase1 | E. coli | 175,000 |
| MBP-paramyosin1 | E. coli | 83,000 |
| MBP-CBD | E. colir | 62,000 |
| Aldolase | rabbit muscle | 47,500 |
| Triosephosphate isomerase | rabbit muscle | 32,500 |
| β-Lactoglobulin A | bovine milk | 25,000 |
| Lysozyme | chicken egg white | 16,500 |
| Aprotinin | bovine lung | 6,500 |
1 MBP = maltose-binding protein. MBP-b-galactosidase = fusion of MBP and beta-galactosidase. MBP-paramyosin = fusion of MBP and paramyosin.
This product is produced at New England Biolabs.
Background
Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands between 6 and 175 kDa when electrophoresed (1). The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDSPAGE gels relative to unstained proteins. The apparent molecular weight of the prestained proteins are shown in the figure below.
Application References
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Companion Products
- 7727 Biotinylated Protein Ladder Detection Pack
This product is produced at New England Biolabs
