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Prestained Protein Marker, Broad Range (Premixed Format) #7720

Description

Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands between 6 and 175 kDa when electrophoresed (1). The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins. The apparent molecular weight of the prestained proteins are given in the table below.

Western Blotting

Western Blotting

10?20% SDS-PAGE

Directions for Use

(a) Transfer the desired amount of the Prestained Protein Marker to a separate tube. For visualizing during electrophoresis: use 15 µl for mini-gels and 30 µl for full length gels.

(b) Heat the Marker to 95-100°C for 3-5 minutes.

(c) After a quick microcentrifuge spin, load directly onto a gel. To ensure uniform mobility, load an equal volume of 1X reducing SDS Loading Buffer into any unused wells. Prestained Protein Marker, Broad Range (Premixed Format) is stable for a minimum of 6 months at -20°C.

Prestained Protein Marker, Broad Range

Protein Source Apparent MW (kDa)
MBP-β-galactosidase1 E. coli 175,000
MBP-paramyosin1 E. coli 83,000
MBP-CBD E. colir 62,000
Aldolase rabbit muscle 47,500
Triosephosphate isomerase rabbit muscle 32,500
β-Lactoglobulin A bovine milk 25,000
Lysozyme chicken egg white 16,500
Aprotinin bovine lung 6,500

1 MBP = maltose-binding protein. MBP-b-galactosidase = fusion of MBP and beta-galactosidase. MBP-paramyosin = fusion of MBP and paramyosin.

This product is produced at New England Biolabs.

Background

Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands between 6 and 175 kDa when electrophoresed (1). The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDSPAGE gels relative to unstained proteins. The apparent molecular weight of the prestained proteins are shown in the figure below.

  1. Laemmli, U. K. et al. (1970) Nature 227, 680-685.

Application References

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This product is produced at New England Biolabs

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