Protein Markers
Prestained Protein Marker, Broad Range (Premixed Format) #7720
Description
Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands between 7 and 175 kDa when electrophoresed (1). The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins. The apparent molecular weight of the prestained proteins are given in the table below.
Western Blotting
Western Blotting
Directions for Use
(a) Transfer the desired amount of the Prestained Protein Marker to a separate tube. For visualizing during electrophoresis: use 15 µl for mini-gels and 30 µl for full length gels.
(b) Heat the Marker to 95-100ºC for 3-5 minutes.
(c) After a quick microcentrifuge spin, load directly onto a gel. To ensure uniform mobility, load an equal volume of 1X reducing SDS Loading Buffer into any unused wells. Prestained Protein Marker, Broad Range (Premixed Format) is stable for a minimum of 6 months at -20ºC.
Prestained Protein Marker, Broad Range
| Protein | Source | Apparent MW (Da) |
|---|---|---|
| MBP-β-galactosidase | E. coli | 175,000 |
| MBP-paramyosin | E. coli | 80,000 |
| MBP-CBD | E. coli | 58,000 |
| CBD-Mxe Intein-2CBD | E. coli | 46,000 |
| CBD-Mxe Intein | E. coli | 30,000 |
| CBD-BmFKBP13 | E. coli | 25,000 |
| Lysozyme | chicken egg white | 17,000 |
| Aprotinin | bovine lung | 7,000 |
This marker was recently calibrated and the revised apparent molecular weights are shown. The formulation of the marker is unchanged.
- CBD
- chitin binding domain
- MBP
- maltose-binding protein.
This product is produced at New England Biolabs.
Application References
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Companion Products
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
