Product Pathways - Kinases
Abl2 Kinase #7729
Cell Signaling Technology offers a full line of protein kinases, substrates, antibody detection reagents and HTScan® kits. Browse our "Reagents for High-Throughput Screening" product listing or contact us at drugdiscovery@cellsignal.com.
Description
Purified recombinant human Abl2 kinase (Glu38-Arg1146) with an amino-terminal His tag.
Source / Purification
The kinase protein was produced using a baculovirus expression system a with construct expressing human Abl2 (Glu38-Arg1146) (GenBank Accession No. NM_005158) with an amino-terminal His tag. The protein was purified by Immobilized Metal Affinity Chromatography (IMAC).
Gel Staining
Figure 1. The purity of the Abl2 protein was analyzed using SDS/PAGE followed by Coomassie stain.
Kinase Assay - Radiometric
Figure 2. Abl2 kinase activity was measured in a radiometric assay using the following reaction conditions: 5 mM MOPS, pH 7.2, 2.5 mM β-glycerophosphate, 1 mM EGTA, 0.4 mM EDTA, 5 mM MgCl2, 0.05 mM DTT, 50 μM ATP, Substrate: Abltide 200 ng/μL, and recombinant Abl2: variable.
Quality Control
The theoretical molecular weight of the Abl2 protein is 132 kDa. The purity of the kinase was assessed using SDS-PAGE followed by Coomassie stain [Fig.1]. Abl2 kinase activity was determined using a radiometric assay [Fig.2].
Background
ARG (Abl-related gene), together with c-Abl, forms the Abl family of mammalian non-receptor tyrosine kinases. ARG and c-Abl share 89%, 90 and 93% identity in their SH3, SH2 and tyrosine domain, but only 29% identity in the carboxy-terminal half. The human c-Abl and ARG genes are expressed ubiquitously. ARG had been detected predominantly in the cytoplasm, whereas c-Abl shows both cytoplasmic and nuclear localization (1). c-Abl is involved in two different chromosomal translocations present in human leukemias, which generate Bcr-Abl and TEL-Abl. Recently, TEL-ARG fusion transcripts have also been identified in acute myeloid leukemias (AML) (2). The Abl family kinases may also interact with receptor tyrosine signaling pathways and regulate cellular function such as cell cycle progression, gene transcription and organization of the actin cytoskeletons in neurons (1,3).
- Scheijen, B. and Griffin, J.D. (2002) Oncogene 21, 3314-33.
- Cazzaniga, G. et al. (1999) Blood 94, 4370-3.
- Yu, H.H. et al. (2001) Oncogene 20, 3995-4006.
Application References
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Companion Products
- 9805 HTScan® Tyrosine Kinase Buffer (4X)
- 9804 ATP (10 mM)
- 7450 Tyrosine Kinase Substrate Screening Kit
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
