Product Pathways - PathScan ELISA
PathScan® Phospho-Insulin Receptor β (Tyr1345) Sandwich ELISA Antibody Pair #7823
|7823S||1 Kit (Reagents for 4 x 96 well plates)||---||In Stock||---|
|7823||carrier free and custom formulation / quantity||email request|
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|Kit Includes||Volume||Cap Color|
|P-Insulin Receptor beta (Y1345) Capture Ab (100X)||0.4 ml||Pink|
|Insulin Receptor betaDetection Ab (100X)||0.4 ml||Blue|
|Anti-mouse IgG, HRP-linked Antibody (1000X)||0.04 ml||Yellow|
Storage: Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.
Important Ordering Details
Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped.
CST's PathScan® Phospho-Insulin Receptor β (Tyr1345) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-Insulin Receptor β (Tyr1345) Sandwich ELISA Kit #7326. Capture and detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The phospho-insulin receptor β (Tyr1345) capture antibody is coated on a 96 well microplate in PBS overnight. After blocking, cell lysates are added followed by an insulin receptor β detection antibody and anti-mouse IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-insulin receptor β (Tyr1345) protein.
Specificity / Sensitivity
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
The relationship between protein concentration of lysates from untreated or insulin-treated CHO-IR/IRS-1 cells and the absorbance at 450 nm using PathScan® Phospho-Insulin Receptor β (Tyr1345) Sandwich ELISA Antibody Pair #7823 is shown. CHO-IR/IRS-1 cells (85% confluence) were serum starved overnight and then treated with insulin (100 nM) for 2 min at 37ºC, and then lysed.
Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).
- Adams, T.E. et al. (2000) Cell. Mol. Life Sci. 57, 1050-1093.
- Baserga, R. et al. (2000) Oncogene 19, 5574-5581.
- Scheidegger, K.J. et al. (2000) J. Biol. Chem. 275, 38921-38928.
- Hernandez-Sanchez, C. et al. (1995) J. Biol. Chem. 270, 29176-29181.
- Lopaczynski, W. et al. (2000) Biochem. Biophys. Res. Commun. 279, 955-960.
- Baserga, R. et al. (1999) Exp. Cell Res. 253, 1-6.
- White, M.F. et al. (1985) J. Biol. Chem. 260, 9470-9478.
- White, M.F. et al. (1988) J. Biol. Chem. 263, 2969-2980.
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- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween® 20 (PBST-20X)
- 9998 BSA
- 7004 TMB Substrate
- 7002 STOP Solution
- 9803 Cell Lysis Buffer (10X)
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7326 PathScan® Phospho-Insulin Receptor β (Tyr1345) Sandwich ELISA Kit
- 3025 Insulin Receptor β (4B8) Rabbit mAb
- 3020 Insulin Receptor β (L55B10) Mouse mAb
- 3026 Phospho-Insulin Receptor β (Tyr1345) (14A4) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
PathScan® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.