Product Pathways - PathScan ELISA
PathScan® Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Antibody Pair #7826
|Kit Includes||Volume||Cap Color|
|PDGF Receptor beta Capture Ab (100X)||0.4 ml||Pink|
|P-PDGF Receptor beta (Y751) Detection Ab (100X)||0.4 ml||Blue|
|Anti-mouse IgG, HRP-linked Antibody||0.04 ml||Yellow|
Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Important Ordering Details
Product is assembled upon order to ensure maximum activity. Domestic: Please allow up to two weeks for your order to be processed and shipped. International: Please allow up to three weeks, depending on the country, for your order to be processed and shipped.
CST's PathScan® Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Kit #7345. Capture and detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The PDGF receptor β capture antibody is coated on a 96 well microplate in PBS overnight. After blocking, cell lysates are added followed by a phospho-PDGF receptor β (Tyr751) detection antibody and anti-mouse IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-PDGF receptor β (Tyr751) protein.
Specificity / Sensitivity
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
The relationship between protein concentration of lysates from untreated or PDGF-treated NIH/3T3 cells and the absorbance at 450 nm using PathScan® Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Antibody Pair #7826 is shown. NIH/3T3 cells (75% confluence) were treated with PDGF (50 ng/ml) at 37°C for 5 minutes, and then lysed.
Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ-mediated PI3 kinase activation (8).
- Deuel, T.F. et al. (1988) Biofactors 1, 213-217.
- Bergsten, E. et al. (2001) Nat. Cell Biol. 3, 512-516.
- Betsholtz, C. et al. (2001) Bioessays 23, 494-507.
- Coughlin, S.R. et al. (1988) Prog. Clin. Biol. Res. 266, 39-45.
- Ostman, A. and Heldin, C.H. (2001) Adv. Cancer Res. 80, 1-38.
- Panayotou, G. et al. (1992) EMBO J. 11, 4261-4272.
- Ramalingam, K. et al. (1995) Bioorg. Med. Chem. 3, 1263-1272.
- Kashishian, A. et al. (1992) EMBO J. 11, 1373-1382.
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- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween 20 (PBST-20X)
- 9998 BSA
- 7004 TMB Substrate
- 7002 STOP Solution
- 9803 Cell Lysis Buffer (10X)
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7345 PathScan® Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Kit
- 3161 Phospho-PDGF Receptor β (Tyr751) Antibody
- 3162 PDGF Receptor β Antibody
- 3166 Phospho-PDGF Receptor β (Tyr751) (88H8) Mouse mAb
- 3169 PDGF Receptor β (28E1) Rabbit mAb
- 3175 PDGF Receptor β (2B3) Mouse mAb
- 4549 Phospho-PDGF Receptor β (Tyr751) (C63G6) Rabbit mAb
- 4564 PDGF Receptor β (C82A3) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.