Product Pathways - PathScan ELISA
PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Antibody Pair #7838
| Kit Includes | Volume | Cap Color |
|---|---|---|
| cdc2 (Tyr 15) Rabbit Capture Antibody (100X) | 0.4 milliliters | Pink |
| cdc2 Mouse Detection Antibody (100X) | 0.4 milliliters | Blue |
| Anti-Mouse IgG, HRP-Linked Antibody (1000X) | 0.04 milliliters | Yellow |
Capture and Detection Antibodies are stored at 4°C. HRP-Linked Secondary Antibody is stored at -20°C.
Species Cross-Reactivity
H
Reactivity Key: H=Human
Description
CST's PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Kit #7176. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The Phospho-cdc2 (Tyr15) Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a cdc2 Detection Antibody and anti-Mouse IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-cdc2 (Tyr15) protein.*Antibodies in this kit are custom formulations specific to the kit.
The relationship between protein concentration of lysates from λ phosphatase-treated and IL-4-treated HeLa cells and the absorbance at 450 nm using PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Antibody Pair #7838 is shown. HeLa cells (80% confluent) were treated with human IL-4 for 10 minutes (100 ng/ml) at 37?C and then lysed.
Background
The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Tyr15 and Thr14 (2). Phosphorylation at Thr14 and Tyr15 resulting in inhibition of cdc2 can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).
- Atherton-Fessler, S. et al. (1994) Mol. Biol. Cell. 5, 989-1001.
- Norbury, C. et al. (1991) EMBO. J. 10, 3321-3329.
- McGowan, C.H. and Russell, P. (1993) EMBO J. 12, 75-85.
- Wells, N.J. et al. (1999) J. Cell. Sci. 112, 3361-3371.
- Hunter, T. (1995) Cell 80, 225-236.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7176 PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Kit
- 4539 Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb
- 9111 Phospho-cdc2 (Tyr15) Antibody
- 9112 cdc2 Antibody
- 9116 cdc2 (POH1) Mouse mAb
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween 20 (PBST-20X)
- 9998 BSA
- 7004 TMB Substrate
- 7002 STOP Solution
- 9803 Cell Lysis Buffer (10X)
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
