Product Pathways - PathScan ELISA
PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Antibody Pair #7861
|Kit Includes||Volume||Cap Color|
|IRS-2 Capture Ab (100X)||0.4 ml||Pink|
|P-Tyrosine Detection Ab (100X)||0.4 ml||Blue|
|Anti-mouse IgG, HRP-linked Antibody (1000X)||0.04 ml||Yellow|
Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Important Ordering Details
Product is assembled upon order to ensure maximum activity. Domestic: Please allow up to two weeks for your order to be processed and shipped. International: Please allow up to three weeks, depending on the country, for your order to be processed and shipped.
CST's PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Kit #7860. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IRS-2 Rabbit Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysate is added followed by a Phospho-Tyrosine Mouse Detection Antibody and an HRP-conjugated, Anti-Mouse IgG Antibody. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-2 phosphorylated on tyrosine.Antibodies in kit are custom formulations specific to kit.
Specificity / Sensitivity
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
The relationship between the protein concentration of the lysate from untreated and insulin-treated CHO (IR/4PS) cells and the absorbance at 450 nm using PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Antibody Pair #7861 is shown. After overnight starvation, CHO (IR/4PS) cells were treated with insulin (100 nM) for 10 minutes at 37ºC and then lysed.
Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).
- Sun, X.J. et al. (1991) Nature 352, 73-77.
- White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.
- Withers, D.J. et al. (1998) Nature 391, 900-904.
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- 7860 PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Kit
- 4502 IRS-2 Antibody
- 3089 IRS-2 (L1326) Antibody
- 9416 Phospho-Tyrosine Mouse mAb (P-Tyr-102)
- 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7004 TMB Substrate
- 7002 STOP Solution
- 9998 BSA
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween 20 (PBST-20X)
For Research Use Only. Not For Use In Diagnostic Procedures.