Cell Signaling Technology

Product Pathways - PathScan ELISA

PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Antibody Pair #7861

Kit Includes Volume Cap Color
IRS-2 Capture Ab (100X) 0.4 ml Pink
P-Tyrosine Detection Ab (100X) 0.4 ml Blue
Anti-mouse IgG, HRP-linked Antibody (1000X) 0.04 ml Yellow

Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Species Cross-Reactivity

M

Reactivity Key:  M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Important Ordering Details

Product is assembled upon order to ensure maximum activity. Domestic: Please allow up to two weeks for your order to be processed and shipped. International: Please allow up to three weeks, depending on the country, for your order to be processed and shipped.

Protocols

Description

CST's PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Kit #7860. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IRS-2 Rabbit Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysate is added followed by a Phospho-Tyrosine Mouse Detection Antibody and an HRP-conjugated, Anti-Mouse IgG Antibody. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-2 phosphorylated on tyrosine.Antibodies in kit are custom formulations specific to kit.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Sandwich ELISA

Sandwich ELISA

The relationship between the protein concentration of the lysate from untreated and insulin-treated CHO (IR/4PS) cells and the absorbance at 450 nm using PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Antibody Pair #7861 is shown. After overnight starvation, CHO (IR/4PS) cells were treated with insulin (100 nM) for 10 minutes at 37ºC and then lysed.

Background

Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).

  1. Sun, X.J. et al. (1991) Nature 352, 73-77.
  2. White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.
  3. Withers, D.J. et al. (1998) Nature 391, 900-904.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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