Product Pathways - Development
AFP (3H8) Mouse mAb (Alexa Fluor® 594 Conjugate) #7877
|IF-IC||H M||Endogenous||Mouse IgG2a|
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
* Product-specific protocol.
Specificity / Sensitivity
AFP (3H8) Mouse mAb (Alexa Fluor® 594 Conjugate) recognizes endogenous levels of total AFP protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human AFP.
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated AFP (3H8) Mouse mAb #3903.
Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma (3).
- Meier, V. et al. (2006) Comp Hepatol 5, 2.
- Warnes, T.W. and Smith, A. (1987) Baillieres Clin Gastroenterol 1, 63-89.
- Peng, S.Y. et al. (2004) Int J Cancer 112, 44-50.
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For Research Use Only. Not For Use In Diagnostic Procedures.