Cell Signaling Technology

Product Pathways - PathScan ELISA

PathScan® Total GFP Sandwich ELISA Antibody Pair #7879

Kit Includes Volume Cap Color
GFP Capture Ab (100X) 0.4 ml Pink
GFP Detection Ab (100X) 0.4 ml Blue
Anti-mouse IgG, HRP-linked Antibody 0.04 ml Yellow

Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Species Cross-Reactivity

Reactivity Key:
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Important Ordering Details

Product is assembled upon order to ensure maximum activity. Domestic: Please allow up to two weeks for your order to be processed and shipped. International: Please allow up to three weeks, depending on the country, for your order to be processed and shipped.

Protocols

Description

CST's PathScan® Total GFP Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total GFP Sandwich ELISA Kit #7878. This antibody pair is intended for use with cotransfected GFP as a convenient means of monitoring transfection efficiency and may not detect GFP fusion proteins. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The GFP rabbit capture antibody is coated onto a 96 well microplate overnight in PBS. After blocking, cell lysate is added followed by a GFP mouse detection antibody and HRP-conjugated, anti-mouse IgG antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of GFP.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Sandwich ELISA

Sandwich ELISA

Figure 1. The relationship between the protein concentration of the lysate from untransfected and GFP transfected HEK 293 cells and the absorbance at 450 nm using the PathScan® Total GFP Sandwich ELISA Antibody Pair #7879 is shown.

Background

The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm (1). Among other applications, GFP is commonly cotransfected alongside a gene of interest as a means of monitoring transfection efficiency.

  1. Miyawaki, A. et al. (2003) Curr Opin Chem Biol 7, 557-62.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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