Product Pathways - Motif Antibodies
Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology) #7902
Description
Cell Signaling Technology's patented PhosphoScan® Technology allows for the purification and identification of phosphorylation sites in cellular proteins when coupled with LC tandem mass spectrometry. In this assay, PhosphoScan® (P-Tyr-100) mAb Beads are used to specifically enrich phospho-tyrosine-containing peptides. Cells are lysed in a urea-containing buffer, and cellular proteins are digested by proteases and fractionated by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using PhosphoScan® (P-Tyr-100) mAb Beads. Overnight incubation ensures high-affinity binding of phospho-tyrosine-containing peptides to P-Tyr-100 beads. Unbound peptides are removed through washing, and phospho-tyrosine-containing peptides are eluted with dilute acid. Reversed-phase chromatography is performed on microtips to separate phosphopeptides from antibody and to concentrate them for LC tandem mass spectrometry.
License / Use Restriction
- Notice to Non-Profit/Academic Purchasers: The purchase of this kit product includes the limited right and license to use the kit in the practice of any technology or methods described and claimed in U.S. Patent Number 7,198,896 and foreign equivalents, “Immunoaffinity Isolation of Modified Peptides from Complex Mixtures”, Rush et al., Pub. Date March 6, 2003 (and equivalents), provided such kit is not used in research or development projects funded in whole or in part by For-Profit/Corporate sponsors or in high-throughput screening projects resulting in information that will be offered to For-Profit/Corporate entities on a fee-for-access basis (which use(s) of purchased kit product, in any field, require a separate commercial use license from Cell Signaling Technology, Inc. ).
- Notice to For-Profit/Corporate Purchasers: The purchase of this kit product does not include the right or license to use such kit in the practice of any technology or methods described and claimed in the U.S. Patent Number named in (i) above. Use of purchased kit product in such methods, in any field, requires a separate commercial use license from Cell Signaling Technology, Inc.
- Notice to all purchasers: The purchase of any other non-kit product, including motif antibody products, from CST does not include the right or license to use such non-kit product in the practice of any technology or method described and claimed in the U.S. Patent Number named in (i) above. Use of purchased non-kit product in such methods, in any field, requires a separate commercial use license from Cell Signaling Technology, Inc.
For license inquiries, please contact Chris Bunker, Ph.D., Director of New Business Development, Cell Signaling Technology, Inc. (phone: 978-867-2307, e-mail: cbunker@cellsignal.com)
Overview
Background
PhosphoScan® Kits, based on CST's published method (1), isolate large numbers of cellular phosphorylated peptides, allowing the investigator to obtain a global overview of phosphorylation in the cell, covering many classes of proteins without preconceived biases about where tyrosine phosphorylation sites will be found.
Tyrosine phosphorylation plays a key role in cellular signaling (2). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (3). Current phosphoproteomic approaches generally reveal only small numbers of tyrosine phosphorylation sites, in keeping with the low level of phosphotyrosine relative to phosphoserine and phosphothreonine residues (4). PhosphoScan® Kit, based on CST's published method (1,5), isolates large numbers of cellular phosphotyrosine-containing peptides. This allows the investigator to obtain a global overview of tyrosine phosphorylation in the cell, covering many classes of proteins without preconceived biases about where tyrosine phosphorylation sites will be found.
- Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
- Schlessinger, J. (2000) Cell 103, 211-25.
- Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
- Mann, M. et al. (2002) Trends Biotechnol 20, 261-8.
- Rush, J. et al. (2003) U.S. Patent Publication , 20030044848.
Application References
- Guo, A. et al. (2008) Proc Natl Acad Sci U S A 105, 692-7. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology) in the following applications: Phosphoscan
- Stokes, M.P. et al. (2007) Proc Natl Acad Sci U S A 104, 19855-60. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology) in the following applications: Phosphoscan
- Rikova, K. et al. (2007) Cell 131, 1190-203. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology) in the following applications: Phosphoscan
- Gu, T.L. et al. (2007) Blood 110, 323-33. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology) in the following applications: Phosphoscan
- Gu, T.L. et al. (2007) Leukemia 21, 563-6. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology) in the following applications: Phosphoscan
- Mercher, T. et al. (2006) Blood 108, 2770-9. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology) in the following applications: Phosphoscan
- Walters, D.K. et al. (2006) Leuk Res 30, 1097-104. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology) in the following applications: Phosphoscan
- Griswold, I.J. et al. (2006) Mol Cell Biol 26, 6082-93. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology) in the following applications: Phosphoscan
- Gu, T.L. et al. (2006) Blood 108, 4202-4. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology) in the following applications: Phosphoscan
- Goss, V.L. et al. (2006) Blood 107, 4888-97. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology) in the following applications: Phosphoscan
- Zhang, Y. et al. (2005) Mol Cell Proteomics 4, 1240-50. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology) in the following applications: Phosphoscan
- Rush, J. et al. (2005) Nat Biotechnol 23, 94-101. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology) in the following applications: Phosphoscan
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