Product Pathways - PathScan ELISA
PathScan® Phospho-Src (Tyr416) Sandwich ELISA Antibody Pair #7963
| Kit Includes | Volume | Cap Color |
|---|---|---|
| Phospho-Src (Tyr416) Rabbit Capture Antibody (100X) | 0.4 ml | Pink |
| Src Mouse Detection mAb (100X) | 0.4 ml | Blue |
| Anti-mouse IgG, HRP-linked Antibody (1000X) | 0.04 ml | Yellow |
Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.
Species Cross-Reactivity
H Dg
Reactivity Key:
H=Human
Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Important Ordering Details
Product is assembled upon order to ensure maximum activity. Domestic: Please allow up to two weeks for your order to be processed and shipped. International: Please allow up to three weeks, depending on the country, for your order to be processed and shipped.
Protocols
- 7963:
- ELISA-P
Description
Cell Signaling Technology's PathScan® Phospho-Src (Tyr416) Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan® Phospho-Src (Tyr416) Sandwich ELISA Kit #7953. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The phospho-Src (Tyr416) rabbit capture antibody is coated onto a 96 well microplate overnight in PBS. After blocking, cell lysates are added followed by a total Src mouse detection antibody and anti-mouse IgG, HRP-linked antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-Src (Tyr416).Antibodies in kit are custom formulations specific to kit.
Specificity / Sensitivity
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Sandwich ELISA
The relationship between the protein concentration of the lysate from untreated and H202-treated MDCK cells and the absorbance at 450 nm using the PathScan® Phospho-Src (Tyr416) Sandwich ELISA Antibody Pair #7963 is shown. MDCK cells were treated with H202 (3 mM) for 3 minutes at 37ºC and then lysed.
Background
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
- Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
- Hunter, T. (1987) Cell 49, 1-4.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7953 PathScan® Phospho-Src (Tyr416) Sandwich ELISA Kit
- 2101 Phospho-Src Family (Tyr416) Antibody
- 2123 Src (32G6) Rabbit mAb
- 9803 Cell Lysis Buffer (10X)
- 7004 TMB Substrate
- 7002 STOP Solution
- 9998 BSA
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween 20 (PBST-20X)
For Research Use Only. Not For Use In Diagnostic Procedures.
