Product Pathways - PathScan ELISA
PathScan® Sandwich ELISA Control Phospho Cell Extract I #7988
Description
Jurkat cells are treated with 100 nM calyculin A and 1 mM pervanadate to inhibit multiple serine/threonine and tyrosine phosphatases, respectively and upregulate protein phosphorylation. Treated Jurkat cells were lysed in 1X cell lysis buffer and lysates were lyophilized.
ELISA
ELISA analysis of PathScan® Sandwich ELISA Control Phospho Cell Extracts I using multiple PathScan® Sandwich ELISA kits. Samples were prepared using the standard ELISA protocol (attached) to a final concentration of 0.25 mg/ml and assayed using the indicated ELISA kits.
Applications
The recommended final concentration for this positive control lysate is 0.25 mg/ml. Reconstitute the lyophilized positive control with 225 μl dH2O to create a 1.5 mg/ml lysate solution. Dilute the sample with 1.0 ml 1X cell lysis buffer (#9803) or a sample diluent supplied with one of our PathScan® Sandwich ELISA kits, to make the final concentraiton at 0.25 mg/ml. Keep on ice. A 100 μL sample should be used in each well of a 96-well plate.
Background
PathScan® Sandwich ELISA is a convenient tool for the analysis of protein expression as well as post-translational modifications (such as phosphorylation and acetylation) allowing the researcher to study signal transduction. PathScan® Sandwich ELISA Control Phospho Cell Extracts I can be used not only to troubleshoot the experiment but also provides the means to standardize the signal allowing the comparison of results obtained from different plates or experiments.
Application References
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Kits and Antibody Pairs
This cell extract can be used as a positive control with a number of PathScan® ELISA kits and antibody pairs. For a listing of compatible products, please see our complete PathScan® ELISA product line.
For Research Use Only. Not For Use In Diagnostic Procedures.
