Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

HELLS Antibody #7998

Applications Reactivity Sensitivity MW (kDa) Source
W H M Mk Endogenous 97 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HELLS Antibody recognizes endogenous levels of total HELLS protein. HELLS Antibody is predicted to detect multiple isoforms of this protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys520 of human HELLS protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HELLS Antibody.

Background

HELLS, or LSH1, is a lymphoid-specific helicase thought to be involved in cellular proliferation and leukemogenesis (1,2). It is believed to be a chromatin remodeler and is required for DNMT1-mediated methylation maintenance and DNMT3A/DNMT3B-mediated de novo methylation. The role of HELLS in methylation maintenance was thought to be largely confined to repetitive DNA sequences, including major and minor satellite sequences, rather than single copy genes (3,4); recent evidence suggests a role in genome-wide cysteine methylation at non-repeat sequences (5). De novo methylation maintenance is associated with silencing of specific genes, some known to be involved in pluripotency and lineage commitment (6,7).

  1. Lee, D.W. et al. (2000) Cancer Res 60, 3612-22.
  2. Fan, T. et al. Epigenetics 3, 134-42.
  3. Dennis, K. et al. (2001) Genes Dev 15, 2940-4.
  4. Muegge, K. (2005) Biochem Cell Biol 83, 548-54.
  5. Tao, Y. et al. (2011) Proc Natl Acad Sci USA 108, 5626-31.
  6. Xi, S. et al. (2009) Stem Cells 27, 2691-702.
  7. Xi, S. et al. (2007) Proc Natl Acad Sci USA 104, 14366-71.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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