Cell Signaling Technology

Product Pathways - Protein Folding

Hip (10B7-C3) Mouse mAb #8014

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 49 Mouse IgG2a

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Hip (10B7-C3) Antibody detects endogenous levels of total Hip (Hsc70-interacting) protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to rat Hip protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Hip (10B7-C3) Mouse mAb.

Background

Hip (HSP70-interacting protein), also known as ST13 (suppression of tumorigenicity protein 13), is one of several co-chaperones that regulate activities of the HSP70 chaperone family (1,2). The homo-oligomeric protein Hip cooperates with HSP70 in protein folding by stabilizing the ADP-bound state of HSP70. Hip directly binds to the ATPase domain of HSP70 when it is converted to the ADP-bound state by proteins of the HSP40 family (3). By collaborating with other positive co-factors such as HSP40 and Hop, or competing with negative co-factors such as Bag1, Hip may facilitate the chaperone function of HSP70 in protein folding and repair, and in controlling the activity of regulatory proteins such as steroid receptors and various regulators of proliferation or apoptosis (4-8).

  1. Prapapanich, V. et al. (1996) Mol. Endocrino.l 10, 420-431.
  2. Gebauer, M. et al. (1997) FEBS Lett. 417, 109-113.
  3. Höhfeld, J. et al. (1995) Cell 83, 589-.98.
  4. Frydman, J. and Höhfeld, J. (1997) Trends Biochem. Sci. 22, 87-92.
  5. Nollen, E.A. et al. (2001) J. Biol. Chem. 276, 4677-4682.
  6. Fan, G.H. et al. (2002) J. Biol. Chem. 277, 6590-6597.
  7. Nelson, G.M. et al. (2004) Mol. Endocrinol. 18, 1620-1630.
  8. Shi, Z.Z. et al. (2007) J. Zhejiang Univ. Sci. B. 8, 170-176.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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