Cell Signaling Technology

Product Pathways - Apoptosis

Fas (4C3) Mouse mAb #8023

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H Endogenous 40-50 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Fas (4C3) Mouse mAb recognizes endogenous levels of total Fas protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human Fas protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with a human Fas construct, using Fas (4C3) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Fas (4C3) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HT-1080 cells using Fas (4C3) Mouse mAb (blue) compared to concentration matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of ACHN cells using Fas (4C3) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers (1,2). Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes (3,4). FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-α, TRAIL, and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites (6). FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26 kDa trimeric soluble form (8,9).

  1. Suda, T. et al. (1993) Cell 75, 1169-78.
  2. Lee, H.O. and Ferguson, T.A. (2003) Cytokine Growth Factor Rev 14, 325-35.
  3. Watanabe-Fukunaga, R. et al. (1992) Nature 356, 314-7.
  4. Hahne, M. et al. (1995) Int Immunol 7, 1381-6.
  5. Nagata, S. (1997) Cell 88, 355-65.
  6. Green, D.R. and Ferguson, T.A. (2001) Nat Rev Mol Cell Biol 2, 917-24.
  7. Walker, P.R. et al. (1997) J Immunol 158, 4521-4.
  8. Kayagaki, N. et al. (1995) J Exp Med 182, 1777-83.
  9. Tanaka, M. et al. (1995) EMBO J 14, 1129-35.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products