Product Pathways - Neuroscience
GGA3 (D66F1) Rabbit mAb #8027
PhosphoSitePlus® protein, site, and accession data: GGA3
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 90 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
GGA3 (D66F1) Rabbit mAb recognizes endogenous levels of total GGA3 protein. This antibody may also recognize cross-reacting bands of unknown origin between 25 and 32 kDa.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu523 of human GGA3 protein.
Western Blotting
Western blot analysis of extracts from rat brain, NIH/3T3 cells, and mouse brain using GGA3 (D66F1) Rabbit mAb.
Western Blotting
Western blot analysis of extracts from H4 cells, untreated, transfected with human GGA3 (hGGA3), or transfected with hGGA3 and treated with Staurosporine #9953 (1 μM, 3 hrs), using GGA3 (D66F1) Rabbit mAb.
Background
GGA3 is a member of the GGA family of proteins which also includes GGA1 and GGA2. These proteins consist of four distinct segments: a VHS domain that binds the di-leucine sorting signal DXXLL; a GAT domain that binds Arf-GTP; a hinge region that recruits clathrin; and a GAE domain that has sequence similarity to γ-adaptin and recruits a number of proteins. Arf1-GTPase recruits GGA3 to the trans-Golgi network. GGAs sort acid hydrolases to the lysosome and are involved in transporting proteins containing the DXXLL signal from the Golgi complex to the endosome (1). During apoptosis or cerebral ischemia, GGA3 is cleaved by caspase-3 at Asp313, reducing GGA3 levels and lysosomal degradation of β-secretase (BACE). The resulting elevated amount and activity of BACE plays a role in amyloid-β (Aβ) production, consistent with BACE elevation and Aβ accumulation in Alzheimer’s Disease (2).
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
