Cell Signaling Technology

Product Pathways - Protein Stability

USP1 (D37B4) Rabbit mAb #8033

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Mk (R) Endogenous 110 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

USP1 (D37B4) Rabbit mAb recognizes endogenous levels of total USP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu768 of human USP1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, A20 and COS cells using USP1 (D37B4) Rabbit mAb.

Background

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action (1,2). There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP1 belongs to the USP subfamily and is regulated in a cell cycle dependent manner by both transcriptional and ubiquitin-proteosomal mechanisms (3). USP1 is a nuclear protein and localizes to chromatin where it is specifically associated with FANCD2. USP1 deubiquitinates monoubiquitinated FANCD2, which plays an important role in DNA damage repair and Chk1 protein stability (3,4). Another important target of USP1 is PCNA. USP1 deubiquitinates monoubiquitinated PCNA, thereby negatively regulating PCNA-mediated translesion synthesis (TLS) during DNA repair (5). USP1 interaction with UAF1, a WD40 repeat-containing protein, leads to formation of an activated USP1/UAF1 complex, which is required for the deubiquitinase activity of USP1 (6,7).

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Nijman, S.M. et al. (2005) Mol Cell 17, 331-9.
  4. Guervilly, J.H. et al. (2011) Hum Mol Genet , .
  5. Huang, T.T. et al. (2006) Nat Cell Biol 8, 339-47.
  6. Cohn, M.A. et al. (2007) Mol Cell 28, 786-97.
  7. Cohn, M.A. et al. (2009) J Biol Chem 284, 5343-51.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products