Cell Signaling Technology

Product Pathways - Apoptosis

DcR2 (D13H4) Rabbit mAb #8049

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 45-60 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DcR2 (D13H4) Rabbit mAb recognizes endogenous levels of total DcR2 protein. This antibody may detect an unidentified protein of 30 kDa in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly270 of human DcR2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct encoding full-length human DcR2 (hDcR2; +), using DcR2 (D13H4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot anlaysis of extracts from various cell lines using DcR2 (D13H4) Rabbit mAb.

Background

The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases.

Death receptor signaling can be controlled by a family of decoy receptors (DcR1, DcR2, and DcR3) that lack a cytoplasmic DD and inhibit death receptor-mediated apoptosis by competing for ligand binding (3-5). Expression of decoy receptors can contribute to chemosensitivity and may provide a mechanism for regulation of apoptosis in certain types of cancer (6-8).

  1. Nagata, S. (1997) Cell 88, 355-365.
  2. Thorburn, A. (2004) Cell. Signal. 16, 139-144.
  3. Sheridan, J.P. et al. (1997) Science 277, 818-21.
  4. Marsters, S.A. et al. (1997) Curr Biol 7, 1003-6.
  5. Pitti, R.M. et al. (1998) Nature 396, 699-703.
  6. Liu, X. et al. (2005) Cancer Res 65, 9169-75.
  7. Spalding, A.C. et al. (2002) Oncogene 21, 260-71.
  8. Bernard, D. et al. (2001) J Biol Chem 276, 27322-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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