Product Pathways - Cytoskeletal Signaling
α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) #8058
PhosphoSitePlus® protein, site, and accession data: TUBA1B
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| F | H M R Mk | Endogenous | Mouse IgG1 |
Applications Key:
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 8058:
- Flow, Immunofluorescence
Specificity / Sensitivity
α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total α-tubulin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with full-length chicken α-tubulin purified from brain extracts. α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate)recognizes residues surrounding Val440.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (DM1A) Mouse mAb #3873.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
