Product Pathways - Cytoskeletal Signaling
α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) #8058
|8058S||100 µl (50 tests)||---||In Stock||---|
|8058||carrier free and custom formulation / quantity||email request|
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|IF-IC||1:100||Human, Mouse, Rat, Monkey||Endogenous||Mouse IgG1|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total α-tubulin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with full-length chicken α-tubulin purified from brain extracts. α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate)
recognizes residues surrounding Val440.
Flow cytometric analysis of Jurkat cells, using α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (red).
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (DM1A) Mouse mAb #3873.
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc., for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.