Product Pathways - Neuroscience
Cleaved GGA3 (Asp313) (D16H3) Rabbit mAb #8068
PhosphoSitePlus® protein, site, and accession data: GGA3
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H Mk | Endogenous | 32 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Cleaved GGA3 (Asp313) (D16H3) Rabbit mAb recognizes endogenous levels of GGA3 protein only when cleaved at Asp313.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp313 of human GGA3 protein.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or treated with staurosporine #9953 (1 μM, 3 hrs), using Cleaved GGA3 (Asp313) (D16H3) Rabbit mAb (upper) and GGA3 Antibody #4167 (lower).
Background
GGA3 is a member of the GGA family of proteins which also includes GGA1 and GGA2. These proteins consist of four distinct segments: a VHS domain that binds the di-leucine sorting signal DXXLL; a GAT domain that binds Arf-GTP; a hinge region that recruits clathrin; and a GAE domain that has sequence similarity to γ-adaptin and recruits a number of proteins. Arf1-GTPase recruits GGA3 to the trans-Golgi network. GGAs sort acid hydrolases to the lysosome and are involved in transporting proteins containing the DXXLL signal from the Golgi complex to the endosome (1). During apoptosis or cerebral ischemia, GGA3 is cleaved by caspase-3 at Asp313, reducing GGA3 levels and lysosomal degradation of β-secretase (BACE). The resulting elevated amount and activity of BACE plays a role in amyloid-β (Aβ) production, consistent with BACE elevation and Aβ accumulation in Alzheimer’s Disease (2).
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
