Cell Signaling Technology

Product Pathways - Metabolism

Succinyl-CoA Synthetase (D8A11) Rabbit mAb #8071

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Mk Endogenous 36 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Succinyl-CoA Synthetase (D8A11) Rabbit mAb detects endogenous levels of total succinyl-CoA synthetase (α subunit) protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human succinyl-CoA synthetase protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Succinyl-CoA Synthetase (D8A11) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Succinyl-CoA Synthetase (D8A11) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Succinyl-CoA synthetase α subunit (SUCLG1) catalyzes the conversion of succinate to succinyl-CoA and plays a key role in the citric acid cycle (1,2). Deficiency of this enzyme leads to a variety of diseases including fatal infantile lactic acidosis (3) and mitochondrial hepatoencephalomyopathy (4).

  1. Johnson, J.D. et al. (1998) J Biol Chem 273, 27580-6.
  2. Phillips, D. et al. (2009) Biochemistry 48, 7140-9.
  3. Ostergaard, E. et al. (2007) Am J Hum Genet 81, 383-7.
  4. Van Hove, J.L. et al. (2010) Pediatr Res 68, 159-64.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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