Cell Signaling Technology

Product Pathways - Adhesion

GIT2 (D11B8) Rabbit mAb #8072

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M Mk Endogenous 85 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GIT2 (D11B8) Rabbit mAb recognizes endogenous levels of total GIT2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GIT2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using GIT2 (D11B8) Rabbit mAb.

Background

G protein-coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac, and cdc42), kinases (such as PAK and MEK), the Rho family GEF Pix, and the focal adhesion protein paxillin (reviewed in 1). GIT1 and GIT2 share many of the same properties, but with at least ten distinct, tissue-specific splice variants. GIT2 has been shown to play an important role in inhibiting focal adhesion turnover and membrane protrusion (2,3). Focal adhesion localization and paxillin binding of GIT2 is regulated through phosphorylation at one or more tyrosine sites (Tyr286, Tyr392, Tyr592) by FAK and/or Src (4,5,reviewed in 6). Once at the focal adhesion, GIT2 is thought to play a key role in cell polarity and migration, making it a protein of interest in the investigation of oncogenic signaling pathways (3,5,7).

  1. Hoefen, R.J. and Berk, B.C. (2006) J Cell Sci 119, 1469-75.
  2. Premont, R.T. et al. (2000) J Biol Chem 275, 22373-80.
  3. Frank, S.R. et al. (2006) EMBO J 25, 1848-59.
  4. Brown, M.C. et al. (2005) Mol Biol Cell 16, 4316-28.
  5. Yu, J.A. et al. (2009) Mol Biol Cell 20, 4706-19.
  6. Yu, J.A. et al. Cell Adh Migr 4, 342-7.
  7. Mazaki, Y. et al. (2006) Nat Immunol 7, 724-31.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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