Cell Signaling Technology

Product Pathways - Neuroscience

TFAM (D5C8) Rabbit mAb #8076

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H Endogenous 24 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TFAM (D5C8) Rabbit mAb recognizes endogenous levels of total TFAM protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val225 of human TFAM protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and A172 cells using TFAM (D5C8) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using TFAM (D5C8) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

TFAM (Transcription Factor A, Mitochondrial; aka TCF6) is a member of the high-mobility group (HMG) proteins because it contains two HMG boxes. TFAM is a transcription factor for mitochondrial DNA (mtDNA), and enhances mtDNA transcription in a promoter-specific fashion in the presence of mitochondrial RNA polymerase and transcription factor B (1). Because the majority of ATP production depends on the mitochondrial respiratory chain, maintenance of the mitochondrial genome is critical for normal health. TFAM plays an essential role in the maintenance of mtDNA and thus, ATP production (2). TFAM binds to mtDNA both nonspecifically and in a sequence-specific manner. It is known to have a dual effect on mtDNA: protection of mtDNA and initiation of transcription from mtDNA (3). TFAM attenuates age-dependent impairment of the brain by preventing oxidative stress and mitochondrial dysfunctions in microglia (4).

  1. Kanki, T. et al. (2004) Mol Cell Biol 24, 9823-34.
  2. Kang, D. et al. Mitochondrion 7, 39-44.
  3. Watanabe, A. et al. (2011) Cardiovasc Res, Epub ahead of print.
  4. Hayashi, Y. et al. (2008) J Neurosci 28, 8624-34.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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