Cell Signaling Technology

Product Pathways - NF-kB Signaling

IFI16 Antibody #8079

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 85, 95, 98 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IFI16 Antibody recognizes endogenous levels of total IFI16 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro399 of human IFI16 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IFI16 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HL-60 cells, untreated (-) or treated (+) with Human Interferon-γ (hIFN-γ) #8901 (100 ng/mL, 16 hr), using IFI16 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

IFI16 belongs to the PYHIN family, which consists of four members in humans (Aim2, IFIX, MNDA, and IFI16) and at least thirteen members in mice (1). The closest homolog of IFI16 in mice is p204. The PYHIN family is characterized by the presence of one or two HIN-200 domains that bind double stranded DNA and a PYRIN domain that mediates interactions among family members, as well as the inflammasome-forming ASC protein (1). IFI16 also has a nuclear localization signal, but has been observed to bind DNA in both the cytoplasm and nucleus (2,3). There are three isoforms of IFI16 produced by alternative splicing (4). IFI16 is constitutively expressed by some cells, such as lymphocytes, and highly inducible in many others by interferons (5). IFI16 has been shown to directly interact with p53 and to play a role in cellular senescence by modulating p53 activity (6). Recently, IFI16 was shown to directly sense intracellular viral DNA, leading to recruitment of STING and activation of IRF-3 and NF-κB (2). Investigators have observed increased levels of IFI16 and anti-IFI16 autoantibodies in patients with systemic lupus erythematosus (7-9).

  1. Keating, S.E. et al. (2011) Trends Immunol 32, 574-81.
  2. Unterholzner, L. et al. (2010) Nat Immunol 11, 997-1004.
  3. Kerur, N. et al. (2011) Cell Host Microbe 9, 363-75.
  4. Johnstone, R.W. et al. (1998) Biochemistry 37, 11924-31.
  5. Trapani, J.A. et al. (1992) Immunogenetics 36, 369-76.
  6. Johnstone, R.W. et al. (2000) Oncogene 19, 6033-42.
  7. Choubey, D. and Panchanathan, R. (2008) Immunol Lett 119, 32-41.
  8. Mondini, M. et al. (2006) Arthritis Rheum 54, 3939-44.
  9. Seelig, H.P. et al. (1994) Arthritis Rheum 37, 1672-83.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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