Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Gelsolin Antibody #8090

Applications Reactivity Sensitivity MW (kDa) Source
W H (Mk) Endogenous 83 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Gelsolin Antibody recognizes endogenous levels of total gelsolin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val377 of human gelsolin protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Gelsolin Antibody.

Background

Gelsolin (actin-depolymerizing factor, ADF, AGEL, Brevin) is an 83 kDa protein that shares structural and functional homology to villin and adseverin/scinderin (1,2). Gelsolin plays an important role in actin filament assembly by capping and severing actin proteins in a Ca2+-dependent manner (3,4). Gelsolin is important for cellular events (e.g., membrane ruffling, chemotaxis, ciliogenesis) that require cytoskeletal remodeling (3); accordingly, cells from gelsolin knockout mice exhibit motility defects, including a failure to ruffle in response to growth factor stimulation (5,6). In humans, defects in gelsolin have been linked to amyloidosis type 5 (AMYL5), a hereditary disease characterized by cranial neuropathy, which appears to result from gelsolin amyloid deposition (7).

  1. Vitale, M.L. et al. (1991) J Cell Biol 113, 1057-67.
  2. Lueck, A. et al. (1998) J Cell Sci 111 ( Pt 24), 3633-43.
  3. Sun, H.Q. et al. (1999) J Biol Chem 274, 33179-82.
  4. Li, G.H. et al. (2010) Med Res Rev, Epub ahead of print.
  5. Azuma, T. et al. (1998) EMBO J 17, 1362-70.
  6. Lu, M. et al. (1997) J Cell Biol 138, 1279-87.
  7. Haltia, M. et al. (1990) Biochem Biophys Res Commun 167, 927-32.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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