Cell Signaling Technology

Product Pathways - Neuroscience

CASK (D14E3) Rabbit mAb #8096

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Endogenous 105 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CASK (D14E3) Rabbit mAb recognizes endogenous levels of total CASK protein. This antibody also detects a background band of unknown origin at 75 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly233 of human CASK protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain and rat brain using CASK (D14E3) Rabbit mAb.

Background

CASK is an adaptor protein with a calcium/calmodulin-dependent protein kinase domain, an SH3 domain, a guanylate kinase homology domain (GUK), and a PDZ domain. CASK links transmembrane proteins to the cytoskeleton and signaling molecules. In particular, CASK binds to neurexin to stabilize pre- and post-synaptic structures (1). While most CASK protein (~80%) is cytoplasmic, a portion of the protein enters the nucleus, where it acts as a transcriptional coactivator (2). Transgenic mice with CASK insertional mutations die within 24 hours of birth (3).

  1. Hata, Y. et al. (1996) J Neurosci 16, 2488-94.
  2. Hsueh, Y.P. et al. (2000) Nature 404, 298-302.
  3. Laverty, H.G. and Wilson, J.B. (1998) Genomics 53, 29-41.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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