Product Pathways - PI3K / Akt Signaling
SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101
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Each control slide contains formalin fixed, paraffin-embedded LNCaP cells, both untreated and treated with PI3-Kinase inhibitor LY294002, that serve as a control for Phospho-Akt (Ser473) immunostaining. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the LY942002 treatment.
Western blot analysis of extracts from LNCaP cells, untreated or treated with LY294002, using Akt Antibody #9272 (upper) orPhospho-Akt (Ser 473) (193H121) Rabbit mAb #4058 (lower). This assay serves as a control for the efficacy of the LY94002 treatment.
Immunohistochemical analysis of paraffin-embedded LNCaP cells untreated (left) or treated with LY294002 (right) using Phospho-Akt (Ser473) (736E11) Rabbit mAb (IHC Specific) #3787.
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or treated with LY294002 (right) using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060.
These slides are intended for use in immunohistochemical assays. Please see the Companion Products for the products that can be used with these slides.
- 4060 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb
- 3787 Phospho-Akt (Ser473) (736E11) Rabbit mAb
- 1140 Phospho-Akt (Ser473) Blocking Peptide
- 4691 Akt (pan) (C67E7) Rabbit mAb
- 4685 Akt (pan) (11E7) Rabbit mAb
- 2920 Akt (pan) (40D4) Mouse mAb
- 2938 Akt1 (C73H10) Rabbit mAb
- 1085 Akt (pan) Blocking Peptide
- 2855 Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
- 9644 4E-BP1 (53H11) Rabbit mAb
- 2997 Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb
- 2691 PRAS40 (D23C7) XP® Rabbit mAb
- 4858 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb
- 2211 Phospho-S6 Ribosomal Protein (Ser235/236) Antibody
- 4857 Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb
- 1220 Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide
- 5364 Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb
- 2217 S6 Ribosomal Protein (5G10) Rabbit mAb
- 2317 S6 Ribosomal Protein (54D2) Mouse mAb
- 1155 S6 Ribosomal Protein Blocking Peptide
- 9323 Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb
- 9315 GSK-3β (27C10) Rabbit mAb
- 5482 Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb
- 11899 Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb
- 5196 NDRG1 Antibody
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8125 SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
- 8059 SignalStain® DAB Substrate Kit
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 5425 Normal Goat Serum
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).
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For Research Use Only. Not For Use In Diagnostic Procedures.