Product Pathways - MAPK Signaling
SignalSlide® Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Controls #8103
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Each control slide contains formalin fixed, paraffin-embedded NIH/3T3 cells, treated with either U0126 (1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene) #9903 or TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 , that serve as a control for phospho-p44/42 MAPK (Thr202/Tyr204) immunostaining. U0126 has been shown to be a highly selective inhibitor of MEK1 and MEK2. TPA induces phosphorylation of p44/42 MAPK. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the U0126 and TPA treatments.
Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, treated with U0126 #9903 (left) or TPA #4174 (right), using Phospho-p44/42MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370.
These slides are intended for use in immunohistochemical assays. Please see the Companion Products for a list of prodcucts that can be used with these slides.
- 4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb
- 4376 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb
- 1150 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Blocking Peptide
- 4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
- 1240 p44/42 MAPK (Erk1/2) Blocking Peptide (#4695 Specific)
- 4696 p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb
- 1245 p44/42 MAPK (Erk1/2) Blocking Peptide (#4696 Specific)
- 9102 p44/42 MAPK (Erk1/2) Antibody
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8125 SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
- 8059 SignalStain® DAB Substrate Kit
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 5425 Normal Goat Serum
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.
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For Research Use Only. Not For Use In Diagnostic Procedures.