Product Pathways - MAPK Signaling
SignalSlide™ Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Controls #8103
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Description
Each control slide contains formalin fixed, paraffin-embedded NIH/3T3 cells, both untreated and treated with Phorbol-13-Myristate-12-Acetate (PMA), that serve as a control for Phospho-p44/42 MAPK (Thr202/Tyr204) immunostaining. PMA induces phosphorylation of p44/42 MAPK. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the the PMA treatment.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells untreated or treated with Phorbol-12-Myristate-13-Acetate (PMA), using Phospho-p44/42 MAPK (Thr 202/Tyr204) (20G11) Rabbit mAb (IHC Preferred) #4376 (upper) or p44/42 MAPK (137F5) Rabbit mAb #4695 (lower). This assay serves as a control for the efficacy of the PMA treatment.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or treated with Phorbol-12-Myristate-13-Acetate (PMA) (right), using Phospho-p44/42MAPK (Thr202/Tyr204) (20G11) Rabbit mAb (IHC Preferred) #4370.
Applications
These slides are intended for use in immunohistochemical assays. Please see the Companion Products for a list of prodcucts that can be used with these slides.
Companion Products
- 4370 Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb
- 4376 Phospho-p44/42 MAPK (Thr202/Tyr204) (20G11) Rabbit mAb
- 8110 Growth/Proliferation Marker: SignalStain® Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Detection Kit
- 1150 Phospho-p44/42 MAPK (Thr202/Tyr204) Blocking Peptide
- 4695 p44/42 MAP Kinase (137F5) Rabbit mAb
- 1240 p44/42 MAP Kinase Blocking Peptide (#4695 Specific)
- 4696 p44/42 MAP Kinase (L34F12) Mouse mAb
- 1245 p44/42 MAP Kinase Blocking Peptide (#4696 Specific)
- 9102 p44/42 MAP Kinase Antibody
- 9108 p42 MAP Kinase (Erk2) Antibody
- 8112 SignalStain® Antibody Diluent
Background
p44/42 MAPK (Erk1 and Erk2), SAPK/JNK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation and control of cellular responses to cytokines and stress.p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK).SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase.
- Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139.
- Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.
- Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.
- Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
- Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.
Application References
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