Cell Signaling Technology

Product Pathways - MAPK Signaling

SignalSlide™ Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Controls #8103

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Description

Each control slide contains formalin fixed, paraffin-embedded NIH/3T3 cells, both untreated and treated with Phorbol-13-Myristate-12-Acetate (PMA), that serve as a control for Phospho-p44/42 MAPK (Thr202/Tyr204) immunostaining. PMA induces phosphorylation of p44/42 MAPK. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the the PMA treatment.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells untreated or treated with Phorbol-12-Myristate-13-Acetate (PMA), using Phospho-p44/42 MAPK (Thr 202/Tyr204) (20G11) Rabbit mAb (IHC Preferred) #4376 (upper) or p44/42 MAPK (137F5) Rabbit mAb #4695 (lower). This assay serves as a control for the efficacy of the PMA treatment.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or treated with Phorbol-12-Myristate-13-Acetate (PMA) (right), using Phospho-p44/42MAPK (Thr202/Tyr204) (20G11) Rabbit mAb (IHC Preferred) #4370.

Applications

These slides are intended for use in immunohistochemical assays. Please see the Companion Products for a list of prodcucts that can be used with these slides.

Companion Products

Background

p44/42 MAPK (Erk1 and Erk2), SAPK/JNK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation and control of cellular responses to cytokines and stress.p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK).SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase.

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