Product Pathways - Apoptosis
SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls #8104
|8104S||1 Pack (5 slides)||---||In Stock||---|
|8104||carrier free and custom formulation / quantity||email request|
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Each control slide contains formalin fixed, paraffin-embedded Jurkat cells, both untreated and treated with etoposide, that serve as a control for cleaved caspase-3 (Asp 175) immunostaining. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the etoposide treatment.
To be used with antibodies: 9664, 9661, 9662, 2035, 9541.
Western blot analysis of extracts from Jurkat cells, untreated or treated with etoposide, using Cleaved Caspase-3 (Asp 175) (5A1) Rabbit mAb #9664 (upper) or Caspase-3 (8G10) Rabbit mAb #9665 (lower). This assay serves as a control for the efficacy of the etoposide treatment.
Immunohistochemical anaysis of paraffin-embedded Jurkat cells, untreated (left) or etoposide-treated (right), using Cleaved Caspase-3 (Asp175) Antibody #9661.
These slides are intended for use in immunohistochemical assays. Please see the Companion Products for a list of products that can be used with these slides.
- 9661 Cleaved Caspase-3 (Asp175) Antibody
- 9579 Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb
- 9664 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb
- 1050 Cleaved Caspase-3 (Asp175) Blocking Peptide
- 9662 Caspase-3 Antibody
- 2035 Cleaved Lamin A (Small Subunit) Antibody
- 5625 Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb
- 8109 SignalStain® Proliferation/Apoptosis IHC Sampler Kit
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8059 SignalStain® DAB Substrate Kit
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 5425 Normal Goat Serum
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).
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For Research Use Only. Not For Use In Diagnostic Procedures.
SignalSlide® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.