Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

SignalSlide® Phospho-Stat1/3/5 IHC Controls #8105

Protocols

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Description

Each control slide contains formalin fixed, paraffin-embedded HeLa cells, untreated, treated with Human Interferon-α1 (hIFN-α1) #8927 or treated with Human Epidermal Growth Factor (hEGF) #8916 that serve as a control for Phospho-Stat1 (Tyr701), Phospho-Stat3 (Tyr705) and Phospho-Stat5 (Tyr694) immunostaining. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the hIFN-α1 and hEGF treatments. SignalSlide® Phospho-Stat1/3/5 IHC Control slides have not been tested as controls for Phospho-Stat1 (Ser727) or Phospho-Stat3 (Ser727) immunostaining.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, untreated (left), treated with Human Interferon-α1 (hIFN-α1) #8927 (middle), or treated with Human Epidermal Growth Factor (hEGF) #8916 (right), using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 (top), Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (middle), or Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 (bottom).

Schematic

Schematic

Applications

These slides are intended for use in immunohistochemical assays. Please see our Companion Products list for products that can be used with these slides.

Companion Products

Background

Stat proteins serve as transcription factors in growth and survival pathways stimulated by growth factor and cytokine activation of receptor proteins. Receptor activation promotes tyrosine phosphorylation of Stat proteins, resulting in Stat dimerization and translocation to the nucleus where they regulate expression of numerous proteins that control cell growth, survival, differentiation and pathogen resistance (1). Stat1 is essential in IFN-α and IFN-γ stimulated pathways and is abnormally activated in many tumors (2,3). Both Stat1α (91 kDa) and Stat1β (84 kDa) isoforms are activated by IFN-α but only Stat1α responds to IFN-γ. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding (4). Transcription factor Stat3 possesses oncogenic potential and anti-apoptotic activities; a number of human tumors display constitutively activated Stat3 (5,6). Activation of Stat3 follows phosphorylation at Tyr705, resulting in dimerization, nuclear translocation and DNA binding (7). Expression of Stat3α (86 kDa) and Stat3β (79 kDa) isoforms correlates with cell type, ligand and cell maturation stage (8). Phosphorylation of Stat5a at Tyr694 is essential for transcription factor activation (9). Stat5 is activated by interleukins and other cytokines (i.e. CSF1); presence of phosphorylated Stat5 is in some IL-3-treated cells suggests a role in angiogenesis and cell motility (10). Altered Stat5 expression and activity is associated with abnormal cell proliferation and apoptosis in some forms of leukemia (11).

  1. Ihle, J.N. (2001) Curr Opin Cell Biol 13, 211-7.
  2. Bromberg, J. (2002) J Clin Invest 109, 1139-42.
  3. Frank, D.A. (1999) Mol Med 5, 432-56.
  4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
  5. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  6. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  7. Darnell, J.E. (1997) Science 277, 1630-5.
  8. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
  9. Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
  10. Buitenhuis, M. et al. (2004) Int J Biochem Cell Biol 36, 2120-4.
  11. Baśkiewicz-Masiuk, M. et al. (2003) Cell Prolif 36, 265-78.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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