Product Pathways - Akt Signaling
SignalSlide™ PTEN IHC Controls #8106
Custom Ordering Details
Sections are cut freshly upon ordering. Domestic: Please allow up to three business days for your order to be processed. International: Please allow up to one week for your order to be processed.
Description
Each control slide contains formalin fixed, paraffin-embedded LNCaP and NIH/3T3 cell pellets. NIH/3T3 cells express PTEN while LNCaP cells do not express PTEN. Western blot analysis was performed on extracts derived from the same cells to verify PTEN expression.
Applications
These slides are intended for use in immunohistochemical assays. Please see the Companion Products for a list of products that can be used with these slides.
Companion Products
Background
PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382 and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein level and activity (8) and is involved in G protein coupled signaling during chemotaxis (9,10).
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- Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-8211.
- Wu, X. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15587-15591.
- Vazquez, F. et al. (2000) Mol. Cell. Biol. 20, 5010-5018.
- Torres, J. and Pulido, R. (2001) J. Biol. Chem. 276, 993-998.
- Freeman, D.J. et al. (2003) Cancer Cell 3, 117-130.
- Funamoto, S. et al. (2002) Cell 109, 611-623.
- Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.
Application References
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