Buffers
SignalStain® Antibody Diluent #8112
Protocols
- 8112:
- IHC / Paraffin
Description
SignalStain® Antibody Diluent is for use in immunohistochemical assays. It yields superior staining with a number of antibodies. This diluent can improve IHC signal and background; however, it is not compatible with all of Cell Signaling Technology's products that are recommended for use in immunohistochemical assays. Please consult the antibody datasheet to determine if SignalStain® Antibody Diluent is recommended for your product of interest.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma comparing SignalStain® Antibody Diluent (left) to TBST/normal goat serum (right) using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060.
Directions for Use
Use as a primary antibody diluent in immunohistochemical assays. Dilute primary antibody in SignalStain® Antibody Diluent as directed on the antibody datasheet.
Application References
- Rimkunas, V.M. et al. (2012) Clin Cancer Res 18, 4449-57. Applications: IHC-P (paraffin)
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8125 SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
- 8107 SignalStain® Akt Pathway IHC Sampler Kit
- 8109 SignalStain® Proliferation/Apoptosis IHC Sampler Kit
- 8113 SignalStain® Phospho-Stat IHC Sampler Kit
- 8111 SignalStain® Phospho-ErbB Family IHC Sampler Kit
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 5425 Normal Goat Serum
For Research Use Only. Not For Use In Diagnostic Procedures.
