Cell Signaling Technology

Buffers

SignalStain® Antibody Diluent #8112

Protocols

Description

SignalStain® Antibody Diluent is for use in immunohistochemical assays. It yields superior staining with a number of antibodies. This diluent can improve IHC signal and background; however, it is not compatible with all of Cell Signaling Technology's products that are recommended for use in immunohistochemical assays. Please consult the antibody datasheet to determine if SignalStain® Antibody Diluent is recommended for your product of interest.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma comparing SignalStain® Antibody Diluent (left) to TBST/normal goat serum (right) using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060.

Directions for Use

Use as a primary antibody diluent in immunohistochemical assays. Dilute primary antibody in SignalStain® Antibody Diluent as directed on the antibody datasheet.

Application References

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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