Cell Signaling Technology

Secondary Antibodies

SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114

Protocols

Specificity / Sensitivity

SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) can be used to detect rabbit primary antibodies in immunohistochemical assays. Some antibodies may require titration when used with this highly sensitive reagent.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma using Sox2 (D6D9) XP® Rabbit mAb #3579 with biotin-based detection (left) or SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb #3077 with biotin-based detection (left) or SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) (right).

Description

SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) is a ready-to-use solution intended for use in immunohistochemical assays to detect rabbit primary antibodies. SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) is a highly sensitive, one-step, polymer-based detection reagent that is specific for rabbit IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all peroxidase-based substrates.

Directions for Use

Equilibrate SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) to room temperature prior to applying to the slides. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature. Wash slides and develop with any peroxidase-based substrate.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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