Cell Signaling Technology

Secondary Antibodies

SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125

Protocols

Specificity / Sensitivity

SignalStain® Boost IHC Detection Reagent (HRP, Mouse) can be used to detect mouse primary antibodies in immunohistochemical assays. Some antibodies may require titration when used with this highly sensitive reagent.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (1E6C4) Mouse mAb #4893 with biotin-based detection (left) or SignalStain® Boost IHC Detection Reagent (HRP, Mouse) (right).

Description

SignalStain® Boost IHC Detection Reagent (HRP, Mouse) is a ready-to-use solution intended for use in immunohistochemical assays to detect mouse primary antibodies. SignalStain® Boost IHC Detection Reagent (HRP, Mouse) is a highly sensitive, one-step, polymer-based detection reagent that is specific for mouse IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all peroxidase-based substrates.

Directions for Use

Equilibrate SignalStain® Boost IHC Detection Reagent (HRP, Mouse) to room temperature prior to applying to the slides. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature. Wash slides and develop with any peroxidase-based substrate.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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