Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

CDC73 (D38E12) Rabbit mAb #8126

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H R Mk (M) Endogenous 60 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CDC73 (D38E12) Rabbit mAb recognizes endogenous levels of total CDC73 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg407 of human CDC73 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CDC73 (D38E12) Rabbit mAb.

Background

CDC73 (HPRT2) is a putative tumor suppressor protein thought to bind RNA polymerase II to help inhibit cell cycle progression (1,2). Commonly referred to as parafibromin, CDC73 is expressed in endocrine tissues, kidney, heart, and skeletal muscle and is localized to both nuclear and cytoplasmic compartments (3). CDC73 acts as a Wnt signaling regulator as it binds the carboxy-terminal region of β-catenin (4). Mutations in the corresponding gene cause an endocrine disorder known as hyperparathyroidism 2, which is characterized by hypercalcemia, bone resorption, and the development of jaw tumors (5).

  1. Rozenblatt-Rosen, O. et al. (2005) Mol Cell Biol 25, 612-20.
  2. Shi, X. et al. (1997) Mol Cell Biol 17, 1160-9.
  3. Woodard, G.E. et al. (2005) Oncogene 24, 1272-6.
  4. Mosimann, C. et al. (2006) Cell 125, 327-41.
  5. Bradley, K.J. et al. (2006) Clin Endocrinol (Oxf) 64, 299-306.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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