Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Cytoskeletal Signaling

Rab10 (D36C4) XP® Rabbit mAb #8127

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Mk Endogenous 23 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Rab10 (D36C4) XP® Rabbit mAb recognizes endogenous levels of total Rab10 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly190 of human Rab10 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Rab10 (D36C4) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells using Rab10 (D36C4) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Rab10 is a member of the Ras superfamily of small Rab GTPases (1) that interacts with Mss4, myosin V (Va, Vb and Vc), and GDI as it helps mediate sorting among cellular endosomes (2-4). Mutation analysis and GFP-fusion protein expression of Rab10 in MDCK cells determined that Rab10 plays a regulatory role in membrane protein transport between early endosomes and basolateral compartments (5,6). Rab10 associates with the GLUT4 complex as a target for AS160 and is required for insulin-stimulated GLUT4 translocation in adipocytes (7,8).

  1. Chen, Y.T. et al. (1993) Proc Natl Acad Sci USA 90, 6508-12.
  2. Burton, J.L. et al. (1994) EMBO J 13, 5547-58.
  3. Roland, J.T. et al. (2009) J Biol Chem 284, 1213-23.
  4. Chen, Y. et al. (2009) Biochem J 422, 229-35.
  5. Schuck, S. et al. (2007) Traffic 8, 47-60.
  6. Babbey, C.M. et al. (2006) Mol Biol Cell 17, 3156-75.
  7. Sano, H. et al. (2007) Cell Metab 5, 293-303.
  8. Larance, M. et al. (2005) J Biol Chem 280, 37803-13.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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