Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-PZR (Tyr241) (D6F9) Rabbit mAb #8131

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H M R B Endogenous 30-50 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-PZR (Tyr241) (D6F9) Rabbit mAb recognizes endogenous levels of PZR protein only when phosphorylated at Tyr241.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr241 of human PZR protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from BAE cells, untreated or H2O2-treated (0.3 mM, 30 min) and untreated HeLa cells using Phospho-PZR (Tyr241) (D6F9) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of BAE cells, untreated (blue) or H2O2-treated (green), using Phospho-PZR (Tyr241) (D6F9) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of BAE cells, untreated (left) or H2O2-treated (2 mM, 30 min; right) using Phospho-PZR (Tyr241) (D6F9) Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).


Background

PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms, designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is the main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan syndrome (8).

Upon Con A treatment or H2O2 treatment, two PZR intracellular ITIM tyrosine sites-Tyr241 and Tyr263 are phosphorylated (4,8). Phosphorylation of these two sites facilitates recruitment of SHP-2 to PZR which alters the phosphatase activity of SHP-2 and affects its downstream signaling (5,8,9).

  1. Zhao, Z.J. and Zhao, R. (1998) J Biol Chem 273, 29367-72.
  2. Zhao, R. and Zhao, Z.J. (2000) J Biol Chem 275, 5453-9.
  3. Kusano, K. et al. Endothelium 15, 127-36.
  4. Zhao, R. et al. (2002) J Biol Chem 277, 7882-8.
  5. Zhao, R. et al. (2003) J Biol Chem 278, 42893-8.
  6. Zannettino, A.C. et al. (2003) Biochem J 370, 537-49.
  7. Zhao, R. and Zhao, Z.J. (2003) Biochem Biophys Res Commun 303, 1028-33.
  8. Eminaga, S. and Bennett, A.M. (2008) J Biol Chem 283, 15328-38.
  9. Zhao, R. et al. (2003) J Biol Chem 278, 42893-8.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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