Cell Signaling Technology

Product Pathways - Metabolism

IDH1 (D2H1) Rabbit mAb #8137

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H M R Mk Endogenous 46 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IDH1 (D2H1) Rabbit mAb recognizes endogenous levels of total IDH1 protein. This antibody does not recognize endogenous IDH2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg222 of human IDH1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IDH1 (D2H1) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using IDH1 (D2H1) Rabbit mAb (blue) compared to concentration-matched Rabbit(DA1E) mAb IgG XP® Isotype Control #3900 (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of 293T cells using IDH1 (D2H1) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

IDH1 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG). These enzymes exist in two distinct subclasses that utilize either NAD or NADP+ respectively, as an electron acceptor (1). IDH1 is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. IDH2 and 3 are mitochodrial enzymes that also function in the Krebs cycle. IDH1 is inactivated by phosphorylation at Ser113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock (2,3). IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma (4). IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway (5).

  1. Ramachandran, N. and Colman, R.F. (1980) J Biol Chem 255, 8859-64.
  2. Bennett, P.M. and Holms, W.H. (1975) J Gen Microbiol 87, 37-51.
  3. Hurley, J.H. et al. (1990) Science 249, 1012-6.
  4. Bleeker, F.E. et al. (2009) Hum Mutat 30, 7-11.
  5. Zhao, S. et al. (2009) Science 324, 261-5.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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