Product Pathways - PI3K / Akt Signaling
LAMTOR2/ROBLD3 (D7C10) Rabbit mAb #8145
PhosphoSitePlus® protein, site, and accession data: LAMTOR2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 14 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
LAMTOR2/ROBLD3 (D7C10) Rabbit mAb recognizes endogenous levels of total LAMTOR2/ROBLD3 protein. This antibody may cross-react with LAMTOR1/C11orf59 and LAMTOR3/MAPKSP1.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu31 of human LAMTOR2/ROBLD3 protein.
Western Blotting
Western blot analysis of extracts from various cell lines using LAMTOR2/ROBLD3 (D7C10) Rabbit mAb.
Background
mTORC1 kinase complex is a critical component in the regulation of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids (3,4). The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1 (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, and LAMTOR3/MAPKSP1 has been identified to interact with and recruit the four Rag GTPases to the surface of lysosomes (5).
- Sancak, Y. et al. (2008) Science 320, 1496-501.
- Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
- Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
- Wullschleger, S. et al. (2006) Cell 124, 471-84.
- Sancak, Y. et al. (2010) Cell 141, 290-303.
Application References
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Companion Products
- 8975 LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb
- 8168 LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb
- 4357 RagA (D8B5) Rabbit mAb
- 3830 RagB Antibody
- 9480 RagC (D8H5) Rabbit mAb
- 3360 RagC Antibody
- 5466 RagC (D31G9) XP® Rabbit mAb
- 4470 RagD Antibody
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
