Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

RagB (D18F3) Rabbit mAb #8150

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 40 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RagB (D18F3) Rabbit mAb recognizes endogenous levels of total RagB protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RagB protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RagB (D18F3) Rabbit mAb.

Background

The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2).

  1. Sancak, Y. et al. (2008) Science 320, 1496-501.
  2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
  3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
  4. Wullschleger, S. et al. (2006) Cell 124, 471-84.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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