Product Pathways - Neuroscience
GFAP (GA5) Mouse mAb (Alexa Fluor® 594 Conjugate) #8152
PhosphoSitePlus® protein, site, and accession data: GFAP
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-F | H M R | Endogenous | Mouse IgG1 |
Applications Key:
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 8152:
- Immunofluorescence
Specificity / Sensitivity
GFAP (GA5) Mouse mAb (Alexa Fluor® 594 Conjugate) detects endogenous levels of total GFAP protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with native GFAP purified from pig spinal cord.
IF-F
Confocal Immunofluorescent analysis of rat cerebellum using GFAP (GA5) Mouse mAb (Alexa Fluor® 594 Conjugate) (red) and Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 488 Conjugate) #8024 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in rat cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated GFAP (GA5) Mouse mAb #3670.
Background
The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system (3).
- Eng, L.F. et al. (2000) Neurochem. Res. 25, 1439-51.
- Goebel, H.H. et al. (1987) Acta. Histochem. Suppl. 34, 81-93.
- Jessen, K.R. et al. (1990) Development 109, 91-103.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
