Cell Signaling Technology

Product Pathways - Protein Stability

USP14 Antibody #8159

Applications Reactivity Sensitivity MW (kDa) Source
W H M R (B) (Dg) (Pg) (Hr) Endogenous 60 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine  Dg=Dog  Pg=Pig  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

USP14 Antibody recognizes endogenous levels of total USP14 protein. Based on sequence alignment, this antibody is predicted to cross-react with both isoform a and isoform b of USP14.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human USP14 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using USP14 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a DDK-tagged cDNA expression construct encoding full-length isoform a of human USP14 (hUSP14-DDK, +), using USP14 Antibody.

Background

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. In humans, there are three proteasomal DUBs: PSMD14 (POH1/RPN11), UCH37 (UCH-L5), and Ubiquitin-Specific Protease 14, which is also known as the 60 kDa subunit of tRNA-guanine transglycosylase (USP14/TGT60 kDa). USP14 is recruited to the proteasome through its reversible association with the PSMD2 (S2/hRPN1) subunit of the 19S regulatory particle. Whereas PSMD14 appears to promote substrate degradation (3,4), USP14 is thought to antagonize substrate degradation (5-8). While the underlying mechanism for the opposing roles of these two proteasomal DUBs is still uncertain, it is thought that USP14 removes ubiquitin from substrate upon docking of the substrate with the 26S proteasome. Furthermore, USP14 trims ubiquitin residues from the distal end of the polyubiquitin chain, thus decreasing the affinity of the chain for the ubiquitin receptors of the proteasome, and allowing for enhanced substrate stability (6,9,10). Studies have elucidated a physiologic role for USP14 in regulating synaptic activity in mammals (11). Research studies have shown that targeting this activity with small molecule inhibitors has potential benefits for the treatment of neurodegenerative diseases and cancer (5,12).

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Verma, R. et al. (2002) Science 298, 611-5.
  4. Yao, T. and Cohen, R.E. (2002) Nature 419, 403-7.
  5. Lee, B.H. et al. (2010) Nature 467, 179-84.
  6. Lam, Y.A. et al. (1997) Nature 385, 737-40.
  7. Koulich, E. et al. (2008) Mol Biol Cell 19, 1072-82.
  8. Jacobson, A.D. et al. (2009) J Biol Chem 284, 35485-94.
  9. Hanna, J. et al. (2006) Cell 127, 99-111.
  10. Thrower, J.S. et al. (2000) EMBO J 19, 94-102.
  11. Wilson, S.M. et al. (2002) Nat Genet 32, 420-5.
  12. D'Arcy, P. et al. (2011) Nat Med, Epub ahead of print.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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