Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Sec23A Antibody #8162

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 82 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Sec23A Antibody recognizes endogenous levels of total Sec23A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe717 of human Sec23A protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Sec23A Antibody.

Background

There are two isoforms of Sec23 protein: Sec23A and Sec23B. Both isoforms have been shown in the Sec23/24 complex, which is a component of COPII coat (1). COPII is composed of at least five proteins: the Sec23/24 complex, the Sec13/31 complex, and Sar1. COPII coat is located at the ER/Golgi interface and involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (2). COPII formation is initiated through binding of the activated G protein, Sar1, to the Sec23/24 complex to form a prebudding complex, which directly binds target molecules (2-4). The prebudding complex further recruits Sec13/31 to form mature COPII coat (5,6). In addition to being a COPII component, Sec23 has also been shown to interact with p125 and Sec16 at the transitional ER; these interactions are important for regulation of the COPII transportation function (7,8).

  1. Paccaud, J.P. et al. (1996) Mol Biol Cell 7, 1535-46.
  2. Aridor, M. et al. (1998) J Cell Biol 141, 61-70.
  3. Miller, E.A. et al. (2003) Cell 114, 497-509.
  4. Mossessova, E. et al. (2003) Cell 114, 483-95.
  5. Barlowe, C. et al. (1994) Cell 77, 895-907.
  6. Bi, X. et al. (2007) Dev Cell 13, 635-45.
  7. Iinuma, T. et al. (2007) J Biol Chem 282, 17632-9.
  8. Hughes, H. et al. (2009) J Cell Sci 122, 2924-34.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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