Cell Signaling Technology

Product Pathways - Metabolism

PFKP (D4B2) Rabbit mAb #8164

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 80 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PFKP (D4B2) Rabbit mAb recognizes endogenous levels of total PFKP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PFKP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PFKP (D4B2) Rabbit mAb.

Background

Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis (1). There are three isozymes: muscle-type, liver-type, and platelet-type (2,3). Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (4,5). Research studies have shown that genetic variations in PFKP are associated with individuals born small for gestational age that are prone to obesity and diabetes later in adulthood (6).

  1. Mediavilla, D. et al. (2008) J Biochem 144, 235-44.
  2. Eto, K. et al. (1994) Biochem Biophys Res Commun 198, 990-8.
  3. Hannemann, A. et al. (2005) Gene 345, 237-47.
  4. Morrison, N. et al. (1992) Hum Genet 89, 105-6.
  5. Vora, S. (1983) Isozymes Curr Top Biol Med Res 11, 3-23.
  6. Morgan, A.R. et al. (2010) BMC Med Genet 11, 125.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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