Cell Signaling Technology

Product Pathways - Metabolism

Hexokinase II (3D7) Mouse mAb #8169

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous 102 Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Hexokinase II (3D7) Mouse mAb recognizes endogenous levels of total hexokinase II protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly332 of human hexokinase II protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Hexokinase II (3D7) Mouse mAb.

Background

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

  1. Warburg, O. (1956) Science 123, 309-314.
  2. Semenza, G.L. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 71-103.
  3. Smith, T.A. (2000) Br. J. Biomed. Sci. 57, 170-178.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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