Product Pathways - Growth Factors/Cytokines
Human Interleukin-7 (hIL-7) #8170
PhosphoSitePlus® protein, site, and accession data: IL7
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Source
Recombinant human IL-7 (hIL-7) Asp26-His177 (Accession #NP_000871) was produced in E. coli at Cell Signaling Technology.
Molecular Characterization
Recombinant hIL-7 has a Met on the amino terminus and has a calculated MW of 17,518. DTT-reduced and non-reduced protein migrate as 17 kDa polypeptides. The expected amino-terminal MDCDI of recombinant hIL-7 was verified by amino acid sequencing.
Purity
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-7. All lots are greater than 98% pure.
Bioactivity
The bioactivity of recombinant hIL-7 was determined in a 2E8 cell proliferation assay. The ED50 of each lot is between 90-1000 pg/ml.
Coomassie Gel
The purity of recombinant hIL-7 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-7 and staining overnight with Coomassie Blue.
Bioactivity
The proliferation of 2E8 cells treated with increasing concentrations of hIL-7 was assessed. After 48 hour treatment with hIL-7, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.
Endotoxin
Less than 0.01 ng endotoxin/1μg hIL-7.
Formulation
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-7. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Background
IL-7 plays a key role in lymphopoiesis and lymphoid homeostasis (1). Stromal and epithelial cells within the bone marrow and thymus produce IL-7 (1). The primary targets of IL-7 are T cells, B cells, and dendritic cells (1). IL-7 is crucial for T cell development, the importance of which is underscored by the lack of T cells in both mice and humans that are deficient in IL-7/IL-7R signaling (1,2). While IL-7 appears to be required for B cell development in mice, the role of IL-7 in human B cell development is unclear (1,3). In addition to its effects on T cell lymphopoiesis, IL-7 promotes the maintenance and survival of naïve and memory αβ T cells, as well as γδ T cells (1). The IL-7 receptor is a heterodimer of the common γ chain, γc, and the IL-7-specific IL-7Rα (1). IL-7 activates PI3K/Akt, Jak1/2, and Stat1, 3, and 5 (1).
- Rochman, Y. et al. (2009) Nat Rev Immunol 9, 480-90.
- Ma, A. et al. (2006) Annu Rev Immunol 24, 657-79.
- Parrish, Y.K. et al. (2009) J Immunol 182, 4255-66.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
