Cell Signaling Technology

Product Pathways - Metabolism

Enolase-2 (D20H2) Rabbit mAb #8171

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 47 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Enolase-2 (D20H2) Rabbit mAb recognizes endogenous levels of total enolase-2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human enolase-2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Enolase-2 (D20H2) Rabbit mAb.

Background

Enolase is a glycolytic enzyme that is involved in the conversion of 2-phosphoglycerate to phosphoenolpyruvate (1). Mammalian enolase has three subunits: α, β, and γ, that can form homo and heterodimers. Homodimers of γ enolase are neuronal-specific (2). Research studies have shown elevated levels of neuro-specific enolase-2 in neuroblastoma (2) and small-cell lung cancer (3,4).

  1. Van Obberghen, E. et al. (1988) J Neurosci Res 19, 450-6.
  2. Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
  3. Stern, P. et al. (2007) Tumour Biol 28, 84-92.
  4. O'Shea, P. et al. (1995) Ir J Med Sci 164, 31-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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